Does Acetaminophen Deplete Glutathione? Understanding the Connection Cymbiotika CYMBIOTIKA Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Sex differences in glutathione metabolism and acetaminophen toxicity Early life acetaminophen exposure, glutathione S transferase genes, and development of adolescent asthma in a high risk birth cohort Journal of Allergy and Clinical Immunology Acetaminophen at low doses depletes airway glutathione and alters respiratory reflex responses Smith 2013 The FASEB Journal Wiley Online Library